Gangadhar Gaikwad

5 Questions 5 Answers 0 Followers

Questions related from Gangadhar Gaikwad

What is the size of band in the well marked by yellow arrow?

I run this gel(1%) and expecting a band of 730bp, but I can't figure out the size of band in the well marked by yellow arrow... so is this the band that I am expecting?

08 August 2018 7,896 2 View

Which 16S reference sequence should I use for Azotobacter chroococcum?

I want to confirm the Azotobacter chroococcum by sequencing it with normal 16s primers, so is there any general sequence available to be used as a reference sequence?

08 August 2018 4,871 0 View

How does azotobacter culture plate smells?

I want to grow azotobacter chroococum, but I found 2 types of colonies...1.crusty and 2.watery (after 10 days incubation). So i separated them and plated on 2 different plates, so only crusty...

05 May 2018 2,095 0 View

Why is smear on gel in my pcr of C-DNA samples?

I am getting smear in the gel of my pcr of CDNA samples, and to surprice me, I am getting band in GAPDH +ve control but not in my CDNA samples... Also I incorporated 2 plasmids as +ve controls,...

12 December 2017 734 2 View

Can anyone help me about multiple bands in my Western blot?

I did Western blot of samples from cotton root tissues from same event, but of different time spans (took samples after 1 month interval for 4 months), and I got difference in banding...

07 July 2014 9,412 7 View