I need to stain frozen sections with 2 different stains (p16 and p21) which has been done in the past on FFPE blocks. Any advice or updated protocol will be much appreciated.
Is anyone researching the relationship between learner engagement and employee engagement? I would be curious to read what you have found on this topic if I haven't already read it. Thanks in advance.
02 March 2024 2,030 0 View
Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?
22 February 2021 3,417 1 View
Dear Martha, I am interested in doing a class to educate nurses how to assess spiritual distress and provide basic spiritual care to patients in the hospital. As part of the class wanted to have...
30 January 2020 9,044 16 View
I am working on horticulture crops, with defined number of farmers, who market at a known formal market. Does anyone have reference to recent work in this direction? What were the lesson and...
17 November 2019 9,434 1 View
WOrking in bariatric clinic, with diabetes managment.
11 July 2019 9,668 5 View
Hi guys as i described in the question, i generated lentivius and i used pGIPz vector from Dharmacon. i checked it's working efficiency on the HEK cell line but after i nasal infect the virus, i...
28 March 2018 8,024 1 View
Dissertation is questioning whether CPR is the main priority in a traumatic cardiac arrest.
24 January 2018 2,601 3 View
I am going to educate staff on health literacy awareness. I will do pre- and post-test. For this, I plan on descriptive statistics and t-test. Is this correct? After beginning advance care...
23 September 2017 7,261 3 View
My curiosity stems from my personal journey as a nurse in transition.
02 July 2017 9,656 3 View
I have a few articles on the Chicago and Boston programs to reduce gun violence and searching for further studies.
14 February 2017 6,666 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View