Hi guys
as i described in the question, i generated lentivius and i used pGIPz vector from Dharmacon. i checked it's working efficiency on the HEK cell line but after i nasal infect the virus, i couldn't find turbo GFP signal in the olfactory epithelium tissue.
Virus titer was not bad.
i wonder is there someone who has similar experience with me.
Thank you for your time
Tammy
Hi girl,
Could you provide more information about your lentivirus preparation protocol?
Because the general lentivirus uses VSV-G for self-packaging. But VSV-G is not an auto skeleton key.
If you want to infect nasal tissue.
1. To increase your lentivirus's titer.
2. To alter the surface protein of pseudo-viral envelope for nasal tissue infection.
(Right now, I only know that the flu virus can easy infect the nasal tissue.)
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