Our laboratory has calculated 146S antigen foot and mouth disease virus for development vaccine is 120 microgram/ml (120 ug/ml). One dose of vaccine is 15 ug of 146S antigen. What buffer can we dilute the antigen in (e.g in water or in PBS) before adding 50% oil.
Thanks so much,
Elvis
Do not quite understand the question, I assume that is the language barrier. However I will try to answer your question.
The first thing I would have liked to know what is the volume of a dose. I assume you got the 146S antigen in the form of IFA (in phosphate buffered saline, or similar) at a concentration of 120 ug / mL. Your need is preparing an oil vaccine that takes into account the volume of mineral oil and that the final formulation has a strength of 15 ug / mL. If this is the case I propose the following, assuming the dose volume is 1 mL:
Vap (aqueous phase) = 0.5 mL
Vop (oily phase) = 0.5 mL
Equation 1 (for IFA need in final formulation)
Vneed = 30 ug/mL * 0.5 mL / 120 mg/mL
Vneed = 0.125 mL
Equation 2 (for determinate the buffer dilution volume)
Vbdv = Vap - Vneed
In general you should dilute the IFA 146S four times to have a corresponding diluted IFA with the necessary strength, in this case 30 ug / mL. Then when the emulsion FABRIQUES 146S IFA diluted twice in the oil and therefore the final formulation will have a strength of 15 ug / mL. My recommendation is that the IFA 146S is diluted with the same buffer currently have, this would avoid possible changes in pH, ionic strength and stability.
I hope I have answered your question. regards
Thank you Carlos Enrique Perez heredia,
I thinking that 146S antigen in dilution "buffer special" and this buffer will high immunology antibodies plus oily phase.
Dose vaccine: (146S antigen dilution in "buffer special") plus (oil) with ratio 1:1
Please help,
Generally I use Phosphate Buffer Saline. I dont know (I dont read) about of any buffer to increase the immunology antibodies. Regards
Usually FMD vaccines are administered as 2 ml doses (mainly for cattle). Therefore you'd require 1 ml from each (aqueous and oil). Phosphate buffer works fine for FMD, however usually 0.06M sodium phosphate buffer containing 0.16M sodium chloride is used for this purpose.
You are welcome - hope this works out for you. Let me know if you've any further questions.
As other colleges said you have to keep the pH of the used buffer at neutral side (i.e. 7.5) because FMD virus is pH labile and will be destroyed above 8 or below 6 pH. So, PBS,normal saline, tissue culture maintenance media can work well.
For an oil FMD vaccine 15 micrograms per ml is quite high + 1 ml is the oil component presuming 2 ml as one dose in cattle and pigs. However, you need to perform duration of immunity and a booster dose 28-30 days after primary vaccination is necessary as it is an inactivated vaccine. Duration also depends on the adjuvant you use plus any other immune boosters.
Does anyone know the common FMD type O, ASIA or A in microgram/per dose for cattle and sheep and goats in quadrivalent of trivalent vaccines ?
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