Why is absorbance measured at 540 nm instead of at 280 nm when quantifying proteins in Lowry and Buiret methods?

Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...

16 December 2020 1,010 3 View

How to heat a reactor up to 900C?

I'm taking a bachelor design, I'm supposed to maintain an endothermic reactor at 900C and 30 bar. The typical jacketed reactor is not really feasible and I'm thinking of fired heater to maintain...

07 October 2020 8,558 5 View

Can you help with method how to compare two emulsions for stability for FAT Oil and grease emulsification?

I'm trying to compare several different surfactant efficiencies for Swage Fato Oil and Grease emulsification into water. I don't think that methods for fat determination in food for example...

06 October 2020 4,941 1 View

What is the importance of need for achievement as a character of entrepreneurs in understanding entrepreneurship?

The three personality characteristics that are documented in almost all entrepreneurship documented information are: i. Need for Achievement ii. Internal Locus of Control iii. Risk-taking Why in...

17 May 2020 9,849 10 View

What is the possible reason why MM calculation of a protein in Spartan suddenly stopped computing?

I am currently running MM (MMFF94) of a protein-ligand complex for geometry optimization using Spartan '18. The calculation went smoothly until the 19919th cycle when it suddenly stopped computing...

19 March 2020 3,628 4 View

A host of papers related to wastewater treatment are published every year, what percentage of these papers are implemented at large scale?

Are the researchers doing enough to assist to the betterment of our society?

03 January 2020 5,554 2 View

Which Statistical Test to use?

Dear all, I have recently conducted an experiment involving the study of how genes respond to varying levels of radiation, and at different time-points after exposure. The experimental conditions...

06 May 2018 5,186 4 View

Can we derive organoids from 2D cell culture?

I am keen to derive organoids from my existing primary tumour cell culture. Is there any existing protocol that you would recommend?

21 March 2018 770 3 View

Why collagen IV solution contain gel after thawed? How to dissolve the gel?

I am using collagen IV to coat flasks for PC12 cells to adhere and grow in serum free supplemented media. After slow thawing, i can see some gel in the collagen IV solution when pipetting up. I am...

10 October 2017 615 2 View

Subject closed. How can i help you?

Subject closed

07 June 2017 5,126 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View