Does anyone have any experiences on using the Thermo Fisher Accucore and Acclaim C30 HPLC columns? Are there any major differences between these two LC columns? I am wanting to separate 2 positional isomers of triglycerides.
I will try to dry a 50% ethanol solution to obtain my dry extract. When I tried to have a 3rd party remove the solvent from my 20mL sample using purely the rotavap, they told me there was...
25 April 2024 5,213 1 View
I will be determining the total phenolic content using Folin Ciocalteu assay. However, I am having difficulty in determining the category of the waste generated. In previous researchers, they...
18 March 2024 421 0 View
A simple conception of critical thinking requirements seems to be easier than the actual practice of the skill. What can be done to shift the whole project such that there are manifest outcomes of...
23 February 2024 6,497 3 View
I am trying to dry my extract using a make-shift vacuum setup with a flask (containing the sample), a solvent trap and a vacuum pump. My heat source would either be a water bath. Can anyone share...
22 February 2024 2,436 1 View
Dear colleagues ! how which diagnostic of differentiating Infected from Vaccinated Animals (DIVA) of the Newcastle disease? Million thanks with Kind regards, Elvis
19 December 2023 6,845 2 View
Recently carried out PCR in order to amplify MMP9 Product from A431 Cells in order to prove its presence in the cancer cell. However, when Electrophoresis was carried out post-PCR here was the...
24 September 2023 5,012 2 View
What could be responsible for the non-amplication of a reference gene in a multiplex assay. The the other two targets amplified but the third (my reference gene) didn't. It however showed...
13 September 2023 2,931 0 View
I am using Taqman Assay Multiplex supplies the expression of 3 genes in my samples. The protocol says for each reach add: Master mix = 5 ul Dye Assay mix (Target 1) = 0.5 ul Dye Assay mix...
11 September 2023 7,772 3 View
According to a CNN article by Jen Christensen, a world-first experiment has been conducted. A human volunteer was given lab-made red blood cells by scientists. According to experts, it has...
22 February 2023 7,053 1 View
I would like to find out if I may get research work on the impact of covid 19 on transport and mobility?
29 March 2022 9,398 11 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View