I’m looking for a way to infiltrating 60-90 nm metallic particles in to muscle tissue from mice. The muscle tissue needs to function as a positive control in a micro-CT imaging setup and the infiltration has to be done on freshly excised tissue.
Electroporation of cells in vitro can accomplish this. EP has proven successful in cancer tumor treatment so you would just need to find the correct parameters to allow the nanoparticles to be internalized. You can also produce xenografts for imaging as well. I would be happy to provide you with citations for both methods.
Thanks for your answer Lisa. EP sounds interesting, I thought this technique was only used on cell cultures and not larger pieces of tissue. A problem might be that some of the particles are charged?
I would be happy to receive citations.
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