How do I refine a specific region of my protein model so that it perfectly superposes with a template peptide PDB with the same sequence?

I have a protein model and want to replace a 12-amino acids-long region within the center of the model with those of a PDB file containing a 12-amino acids long peptide of the same sequence. This...

02 February 2021 779 2 View

Is there any way to output LAMMPS readable data file which can identify similar atoms but in different molecules??

I have been working with a complex system that involves 5 different atoms and 3 different molecules. However 2 of the atoms are present in 2 different molecules i.e O & H. Now when I am...

10 March 2020 4,547 2 View

What is the best way to test the porosity of a scaffold?

The pore size of my developed bone scaffold ranges within 100-500 microns as observed from SEM analysis. What is the best way out of BET and mercury intrusion porosimetry to test the the pore...

19 August 2019 7,324 2 View

I am looking for a sealing materials which can be used at around 1000 celsius and 50 bar pressure. Are there any ceramic which can be gas tight?

I need to seal peripheral part of tube which operates at 1000 degree celsius and 50 bar pressure. So the seal should be gas tight.

10 June 2019 2,792 2 View

Is there any library in python for evaluationary algorithm?

evolutionary algorithm

02 April 2019 8,065 16 View

How to Generate/simulate data from R value and regression equation?

Mean and standard deviation are not given. Only regression/ trend line equation and R value are given. How to simulate 100 nos. data from above scenario?

13 June 2018 7,987 4 View

Could you please suggestion some journal without publication fees?

Engineering, Civil Engineering, Geotechnical Engineering related Journal with mid-to-low impact factor. Please do not preferred Indian journals.

16 March 2018 8,626 31 View

How reactive camphor is?

I am looking for a porogen which is non-reactive (inert) in nature. I want to use camphor as a porogen. So I want to know how reactive camphor is?

26 October 2016 7,108 3 View

What kind of lysis buffer should I use for detecting lc3-II bands on western blot?

I am using these cell lines: HCT116 and HT29 (colorectal cancer cell lines) and BJ (skin fibroblasts) and IEC-6 (small intestine cells from rats).

29 April 2015 8,559 12 View

Can two areas modeled in different cs be displayed at a time in a single wokplane?

Hello everyone, I have two different rectangles modeled in two different cs in ANSY MAPDL. But while displaying in the workplane, the orientation of any of the one cs comes at a time and...

24 February 2015 4,851 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

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17 February 2021 4,217 3 View