275 ul TEA
550 ul phosphoric acid
then adjusted the pH to 3.30 with KOH (10%).
Using a ESI-QIT mobile phase A: water 0,1% FA mobile phase B: ACN 0,1%FA Solved insulin in water with 0,1% FA (5 ug/ml) Up to 10 blancs after injection of the standard shows carry-over at the...
02 March 2018 1,029 9 View
I made a buffer as follow: 275 ul TEA 550 ul phosphoric acid then adjusted the pH to 3.30 with KOH (10%). My peaks came out allmost perfect. I had tailing on one of the 4 components, which is a...
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