Michel Verhoeven

4 Questions 7 Answers 0 Followers

Questions related from Michel Verhoeven

Why do I get carry-over of insulin in LC-MS?

Using a ESI-QIT mobile phase A: water 0,1% FA mobile phase B: ACN 0,1%FA Solved insulin in water with 0,1% FA (5 ug/ml) Up to 10 blancs after injection of the standard shows carry-over at the...

03 March 2018 1,050 9 View

Why is a full scan combined with SIM better for quantification than only SIM?

I measured an intact protein with ESI Ion Trap and got 3 ions with multiple charges. I focussed on the one with the highest relative abundance. I did a full scan with SIM and only a SIM. The area...

22 February 2018 3,930 4 View

How do you call this buffer?

275 ul TEA 550 ul phosphoric acid then adjusted the pH to 3.30 with KOH (10%).

09 September 2017 2,254 0 View

How do I make a buffer with TEA?

I made a buffer as follow: 275 ul TEA 550 ul phosphoric acid then adjusted the pH to 3.30 with KOH (10%). My peaks came out allmost perfect. I had tailing on one of the 4 components, which is a...

09 September 2017 10,024 2 View