I have a friend is trying to clone a specific AFLP marker out of Medicago truncatula. He knows the selective bases and needs for the end of the primers. His problem is that all the AFLP cloning kits he has found are intended for whole genome AFLP mapping. Since he only needs to find one marker, is there a cheaper and easier way to get only one?
Ragupathi Nagarajan
It may be possible by isolating the band and re amplifying before cloning. I remember this from one of my graduate class.
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Silvio Alejandro López-Pazos
You try this: using a micropipette tip pick the gel and put the tip in boiling water (1 min) until gel thaw. Eluate could be use for re ampliying, sequencing and cloning.
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