How do you adjust standard settings and FSC gate for detection of apoptosis using flow cytometry with annexing V/FITC staining?

09 September 2015 1 9K Report

I am inducing apoptosis in HCT-116 cells with Doxorubicin and am not sure how to adjust the initial setting on the flow cytometer.

Gary Warnes

Hi Emily, the problem you have maybe is that doxorubicin is itself fluorescent in the PI channel, so you need to switch viability markers to DAPI or DRAQ7

hope this helps

Gary

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