How do we transform our Ct value to log(N) for input to NormFinder?

29 September 2017 0 1K Report

Dear All,

I am working on determination reference gene for qPCR, I think normfinder is quiet simple software to assay our best reff gene. Whereas we couldnt just simple input our Ct value, but we need to transform to log(N). Can I use my standard curves if I have done my qPCR efficiency for those genes, to get log(N) data for NormFinder?

Thank You

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Science method

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