I want to identify two different individuals of parasitic plants growing in one host from each other. Thing that I know, to do DNA barcoding for species identification we can use mitochondrial cytochrome oxidase I gene (COI). Is there a DNA identification protocol for individual level? Are we using specific genes as markers?
Please let me know if there any reference for it.
As far as I know DNA barcoding is used for species level identification and Mt DNA does not provide much about intrapopulation differences, or more clearly nuclear DNA provides more. Like we do in crimical studies, DNA finferprinting, you have to find one nuclear marker working good for plants.
Dear Artur Burzynski ,
I initially mistook your remark. You are right about Mt DNA. However, the problem with the mitochondrial genome may not just be the speed. Sort of balancing is also implied in some works.
Städler, T. and Delph, L.F. 2002. Ancient mitochondrial haplotypes and evidence for intragenic recombination in a gynodioecious plant. Proceedings of the National Academy of Sciences of the United States of America, 99: 11730-11735.
CpDNA is also slow. You may not get good signals regarding the taxonomy due to high levels of introgression in some groups. However, still you get some information about isolation and taxonomic relationships.
Simeone, M. C., Piredda, R., Papini, A., Vessella, F., and Schirone, B. 2013. Application of plastid and nuclear markers to DNA barcoding of Euro-Mediterranean oaks (Quercus, Fagaceae): problems, prospects and phylogenetic implications, Bot. J. Linn. Soc. 172: 478–499.
Wolfe, K,H, Li, W.H., Sharp, P.M. 1987. Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast and nuclear DNA. Proc Natl Acad Sci. USA, 84: 9054–9058.
Dear Arthur Burzynski,
Thank you very much for the orientation and contribution. I find this kind of discussions helpful for my science, although there may some occasional misunderstandings occur.
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