I've put my miRNA sequence (5'flanking sequence+ silencing + loop + 3' flanking sequence) in UNA Fold from IDT Technologies website and it gave a potential structure of my miR with detlaG values, delta H, deltaS values and also Tm. How can I assess whether my miR is thermodynamically stable based on these values?
what are the ranges for a 'good Tm' and a good delta G value?
Hello
See the links here hope help you
Good Luck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885156/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956623/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452852/
https://pdfs.semanticscholar.org/7678/b9627596aba67584c14471e00f9b85db2f21.pdf
http://www.sciencedirect.com/science/article/pii/S1046202312002551
Dear Valery
According to Ambros (2004) microRNA (abbreviated miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals and some viruses, that functions in RNA silencing and post-transcriptional regulation of gene expression
I suggest you also this paper: Failure of the PAXgene Blood RNA System to maintain mRNA stability in whole blood.
Thank
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956623/
http://www.nature.com/nature/journal/v431/n7006/full/nature02871.html
http://www.cell.com/cell/fulltext/S0092-8674(04)00045-5
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