I want to know why the PLGA nanoparticle prepared by double emulsion method have not stability in water.
I have used 20 mg of PLGA in DCM and acetone and mixed with 1 mg of Doxorubicin-HCl(O/W1) and sonicated for 1 min at 38% amplitude and then added the mixture in 5% PVA and sonicated for 10 min at 88% amplitude. Then this whole mixture is mixed with 10 ml of 0.1% PVA again and stirred for 16 hrs to remove DCM-acetone. Then centrifuged at 18000 RPM for 20 min and washed with water thrice.
The particle is soluble in water or PBS. But gradually settles at the early hours of preparation in room temperature . Can any one please tell me what could have went wrong in this or what can be done to stabilize this?
First of all the particles are dispersable not soluble. Are you using ester or acid terminated plga? What is their size and zeta potential? How long do they take to settle?
they are dispersable, acid endcap polymer, -12.5 in charge and about 190 nm size
When the plga particles settle, can you redisperse them by simple shaking?
I am facing the same problem now please add your answers if you have figured it out. appreciate it
If the particles settle down, the you have more likely microparticles.
Try to filtrate them through 0,45µm. I am sure, they do not pass.
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology