Should lysis buffer be fully removed or should some be left behind during DNA digestion?

I've been having issue with extracting/precipitating DNA from mouse tail pieces and am unsure if the lysis buffer I use could be the problem. After digesting for 2 hours with lysis buffer and...

04 February 2021 1,364 1 View

How we can fuzzify a data using matlab?

I have a set of clinical data and i want to get the fuzzy values of that particular data . could you suggest a Matlab program for it ?

16 January 2021 6,562 3 View

Best way to get air out of a hamilton syringe?

I'm using a hamilton syringe to inject mineral oil through PVC tubing attached to the needle tip of the syringe. To make sure the tubing stays free of bubbles, I have to get all the air out of the...

10 January 2021 8,082 1 View

Will the new vaccines be effective against the new SARS-Cov2 strain from the UK?

Two new variants of the coronavirus were detected in the U.K. and have now been followed to other parts of the world including South Africa and North America. The South African variant could be...

05 January 2021 499 4 View

How to dissociate and culture retinal cells from mouse eyes?

We have a transgenic mouse line in the laboratory from which we are looking to culture retinal cells from. Our experiments indicate we might be able to produce stable cultures from these cells...

03 January 2021 7,200 6 View

How to find repeatable experiments ?

Greetings, I am a Ph.D. student and have the following assignmnet: Research in databases and business intelligence. Your task for this week is to recreate an experiment conducted in a recent...

26 October 2020 3,459 3 View

For a two-way ANOVA, if one of my main effects is not significant, however my interaction effect is, am I still able to run interaction contrasts?

I have set my contrasts up in SPSS, however the output for my interaction contrasts simply says "This L matrix is not estimable. Hypothesis tests cannot be computed." And I am unsure as to whether...

26 September 2020 1,195 3 View

Are. or are not, superinsulators with respect to electricity also superinsulators with respect to heat?

Superconductors with respect to electricity manifest zero resistance to electric current. But they are NOT also superconductors with respect to heat. The flip side of electrical superconductivity...

14 August 2020 2,214 7 View

Affordable EMG equipment?

I'm looking to buy a cheap portable set-up to record surface Electromyography (EMG). Does anyone know if its possible to purchase a device that can record from two muscles for under approx. 500...

05 July 2020 4,780 3 View

What steps do I need to take to conduct a power analysis when using Hayes' PROCESS model number 58?

This is a moderated mediation model, and I have no idea where to start to determine a sample size or how to conduct a power analysis.

07 March 2020 4,928 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View