How to make ribbon protein structure picture?

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What are skewness/kurtosis sampling distributions?

For the skewness and kurtosis coefficients sampling distributions, are they assumed to be normal or some other distribution shape?

15 May 2024 6,037 0 View

How to process CG simulation for protein containing disulfide bonds?

Hello, everyone I am simulating a single chain protein containing disulfide bonds. Force filed of Corase Grained simulation is Martini 2.2, it was run in gromacs2020 in Windows system. I wonder...

07 May 2024 4,213 2 View

I need a reading related to adherence of data protection policies?

challenges affecting adherence as per data protection policies is concerned

21 April 2024 2,654 1 View

How does pymol software render the same structure as ChimeraX?

Hello, everyone! Can somebody help me? I want to draw the protein structure in pymol with this rendering effect, how should I set the light?

17 April 2024 3,378 1 View

Poor DAPI staining in fixed brain sections?

I have a question about some weird nuclei I see while imaging. I'm sorry if this isn't the best place to write it! One image has bad, "shattered"-looking nuclei from dentate gyrus. The other...

08 April 2024 7,534 2 View

How to explain the higher intramolecular FRET efficiency of flexible structures?

The BFP and GFP are connected by a flexible linker and FRET is more higher.

18 March 2024 5,035 4 View

Is there a database for European Carabidae traits?

I am looking for a database of European Carabidae traits - average body length, wing morphology, habitat preference, etc. I have come across Carabids.org but it seems to be down. Is anyone aware...

17 December 2023 4,079 9 View

Request for Literatures ?

Use a model trained in basic theory to recognize the type, location and size of defects. Please help with such papers or articles, web sites. Thank you!

01 December 2023 7,000 0 View

How to formulate acrylic adhesive to bond two plastics?

Common acrylic adhesive, such as two part acrylic adhesive, can bond two metal material very tightly, but can not bond two plastics. Is there any solution to improve the adhesion ability between...

31 October 2023 5,786 1 View

Is this the best way to present a analysis of breaking waves on a breakwater with sea level rise?

I am currently investigating a breakwater and how it will cope with climate change, particularly sea level rise. I have collected wave data and doing a hand calculation method to test the wave...

06 September 2023 5,285 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Element deletion problem in ABAQUS/Explicit solver using Cohesive elements?

I had a problem for so long. I could easily delete elements in ABAQUS implicit solver but I needed to use VUMAT so I had to have Explicit solution but the element did not disappear for so long. I...

23 July 2024 10,032 0 View