How to make ribbon protein structure picture?

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How we can fuzzify a data using matlab?

I have a set of clinical data and i want to get the fuzzy values of that particular data . could you suggest a Matlab program for it ?

16 January 2021 6,562 3 View

Best way to get air out of a hamilton syringe?

I'm using a hamilton syringe to inject mineral oil through PVC tubing attached to the needle tip of the syringe. To make sure the tubing stays free of bubbles, I have to get all the air out of the...

10 January 2021 8,082 1 View

Will the new vaccines be effective against the new SARS-Cov2 strain from the UK?

Two new variants of the coronavirus were detected in the U.K. and have now been followed to other parts of the world including South Africa and North America. The South African variant could be...

05 January 2021 499 4 View

How to dissociate and culture retinal cells from mouse eyes?

We have a transgenic mouse line in the laboratory from which we are looking to culture retinal cells from. Our experiments indicate we might be able to produce stable cultures from these cells...

03 January 2021 7,200 6 View

How to find repeatable experiments ?

Greetings, I am a Ph.D. student and have the following assignmnet: Research in databases and business intelligence. Your task for this week is to recreate an experiment conducted in a recent...

26 October 2020 3,459 3 View

For a two-way ANOVA, if one of my main effects is not significant, however my interaction effect is, am I still able to run interaction contrasts?

I have set my contrasts up in SPSS, however the output for my interaction contrasts simply says "This L matrix is not estimable. Hypothesis tests cannot be computed." And I am unsure as to whether...

26 September 2020 1,195 3 View

Are. or are not, superinsulators with respect to electricity also superinsulators with respect to heat?

Superconductors with respect to electricity manifest zero resistance to electric current. But they are NOT also superconductors with respect to heat. The flip side of electrical superconductivity...

14 August 2020 2,214 7 View

Affordable EMG equipment?

I'm looking to buy a cheap portable set-up to record surface Electromyography (EMG). Does anyone know if its possible to purchase a device that can record from two muscles for under approx. 500...

05 July 2020 4,780 3 View

What steps do I need to take to conduct a power analysis when using Hayes' PROCESS model number 58?

This is a moderated mediation model, and I have no idea where to start to determine a sample size or how to conduct a power analysis.

07 March 2020 4,928 1 View

What are some analytical techniques to analyze data from a moderated mediation model?

I am proposing that social comparisons on social media will mediate the relationship between passive social media use and social anxiety, and that gender will moderate this association, such that...

05 March 2020 7,197 3 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View