How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

What is the best format to export from igraph to gephi?

I have been using R, igraph, and gephi to create semantic networks. I generally export gml file from R through igraph, then import the gml file into gephi for further adjustment. However, I am now...

06 September 2020 3,906 4 View

How to find a transfer function from frequency response data?

I have data from several different frequency responses of the same open-loop system. This data has been put together to plot a Bode plot and Nyquist plot using MATLAB. I now need to find a...

18 April 2020 7,984 4 View

Interactive app or software for creating and testing simple models?

Do you know of any interactive apps that allow users to setup simple 2D simulations, such as: - repulsive gas, - liquids, - brownian motion, - polymer solutions, - etc. ?

01 January 2018 5,054 2 View

WhIch is the best textbook for learning meta-analysis with R?

Dear all: I have experience in meta-analysis using Comprehensive Meta-Analysis software, but a complete novice at meta-analysis with R. Would you please recommend which is the best textbook or...

10 November 2017 1,116 2 View

Is Comprehensive Meta-Analysis Software appropriate for conducting a dose-response meta-regression of cohort studies?

Traditional meta-regression analyses provide the pooling estimates assume independence of the estimates, an assumption that may be not true because the estimates for separate exposure levels...

09 October 2017 4,470 4 View

How do I determine if an intramolecular proton transfer is likely to happen in Gaussian simulation?

03 October 2017 2,448 7 View

Which Cp ring of carboxylic ferrocene can undergo eletrophilic addition?

I use carboxylic ferrocene (Fc-COOH) and react with acetic anhydride in H3PO4. Hopefully I can get acetylation on the Cp ring without COOH. But from H NMR, it seems that I got 100% of acetylation...

14 March 2016 7,918 3 View

Can I use different cells to set up compensation controls for flow cytometer?

I was wondering if I would be able to use BV2 cell line to set up the compensation controls for apoptosis assay on flow cytometer. I am staining the cells with annexin and PI. I would like to...

15 December 2015 8,321 4 View

How would I use phagocytosis assay using e.coli Bioparticles on flow cytometer?

15 December 2015 9,530 7 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View