Dear Experts,
We would like to inquire if planned to buy commercialized Anti-HER2/neu (369-377) T Cells to perform a cytotoxic T Lymphocyte (CTL) assay.
Do we need to use APC to activate T cells before CTL assay?
(Co-culture with antigen+APC first?)
We only have limited experience with CAR-NK cells.
In our experience, CAR-NK could use to kill the target cells.
However, we are not sure whether T cell needs to go through activation as we haven't found a proper protocol for the experiment.
Could you help us to solve the myth?
Sincerely,
Hello Po-Han Chao
CAR-T cells are synthetic molecules in which the effector function of T lymphocytes combines with the ability of antibodies to identify specific antigens. Thus, CAR T cells do not require antigen presentation by antigen presenting cells (APC) and can recognize intact proteins. Therefore, the creation of genetically engineered T cells redirected to tumor antigens can bypass several mechanisms of immunological tolerance.
Please refer to the article attached for more information.
Article Specific activation and targeting of cytotoxic lymphocytes t...
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