Aleks Po

13 Questions 15 Answers 0 Followers

Questions related from Aleks Po

Can somebody help me with a light scatter plot from lysis of peripheral blood?

Im looking at the leukocyte in mouse peripheral blood. Lysing rbcs with ack having some issues with incubation time Tried 10-15min Figure 1 the plot looks different from previous trials ( figure...

19 December 2015 2,592 4 View

Can I use different cells to set up compensation controls for flow cytometer?

I was wondering if I would be able to use BV2 cell line to set up the compensation controls for apoptosis assay on flow cytometer. I am staining the cells with annexin and PI. I would like to...

16 December 2015 8,442 4 View

How would I use phagocytosis assay using e.coli Bioparticles on flow cytometer?

Hi, I would like to use Vybrant phagocytosis assay, using fluorescently labeled e.coli Bioparticles, to measure phagocytosis in BM derived neutrophils. I would like to read the samples on the...

16 December 2015 9,670 7 View

Can somebody help choosing a lysing solution for murine blood?

Working with murine blood to look at the population of the circulation neutrophils on the flow need to lose RBCs and not activate the leukocyte population

16 December 2015 2,625 7 View

What is the optimal culture media for BV2 cells?

Hi, I'm currently using DMEM with 10% HI FBS. Recently seeing no LPS response after treating the cells for 24 hours. I used the media without Hepes. Found the thread that the media needs to be...

03 December 2015 6,013 4 View

Has anybody had issues using absolute counting beads to count cells in mouse peripheral blood?

Hi, I am using Absolute counting beads to count the number of neutrophils in mouse peripheral blood. I was wondering if somebody has done similar work. I am using Count Bright beads from...

30 November 2015 4,604 6 View

How do I stain myeloperoxidase in neutrophil?

I used neutrophil elastase antibody to stain brain sections and got a lot of background staining. I used 3% H2O2 and 2% serum blocking.

08 October 2015 4,272 3 View

What is the best way to isolate neutrophils from bone marrow?

I was wondering what is the best way to isolate neutrophils from the bone marrow without activation. what is the approximate number of cells will I get from one mouse 8-12 weeks? there is a couple...

01 October 2015 3,671 5 View

Can anybody recommend the speed to centrifuge neutrophils?

Hi All, I am having a problem with pelleting down the neutrophils after isolation. I use 300g for 10minutes, but I seem to be loosing most of the cells. Can anybody recommend what I can do to...

09 September 2015 2,911 4 View

Why would Total Tau antibody not work on Western blot?

Hi Colleagues, I just did a western today to look at Phospho Tau and Total Tau protein levels in hippocampus of 3TgAD mice and met some complications [Phospho Tau Pierce MN #1020; Total Tau...

06 December 2014 6,260 2 View

Is anyone familiar with Western Blot Loading?

Hi, I have recently encountered a loading problem with one of my blots. The data just didnt make any sense. I reprobed for actin. The loading problem should be correctable for actin normalization...

29 September 2014 5,963 3 View

Can anyone help me resolve the background issues I've been having with Western blot?

Hi, Recently one of my blots came out looking like this, I am trying to figure out whats causing this problem, the concentration of the antibody or something else. My blotting protocol is 3x...

28 September 2014 881 13 View

Does anybody know how I can correctly adjust the compensation matrix for multicolor flow?

Does anybody know how I can correctly adjust the compensation matrix for multicolor flow. I used the BD Comp beads to set up the matrix in FlowJo, yet it doesn't fully compensate my samples. I...

13 June 2014 9,550 6 View