I am trying to do KASP genotyping using a cfx96 Biorad qPcr. When i tried my first sample the rfu of the negative control, water, was just as strong as my samples and the controls. Later I tried to run a plate with just water and empty wells with the positive controls provided by the kit and the negative wells still had rfus just as strong as the controls. Has anyone else had this problem or have any possible solutions? 

Secondly I was wondering if anybody knew of a cheap really strong positive control to use with the qpcr. My lab has free Bodipy available and I was wondering if that could be used as the control? 

Thanks in advance,

Andrew