Nair Temis Olguin Alderete

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Questions related from Nair Temis Olguin Alderete

Has anybody tried the EasyPure RNA Kit (TRANS) for extracting rna directly from wine?

Hello, I have used Roche RNeasy kit for RNA extractions directly from wine with no problem at all but I am trying this other kit and I am having some difficulties. Has anybody tried the EasyPure...

24 January 2018 5,517 3 View

Is there a difference when extracting RNA from different varieties of wines?

Hello, I have extracted RNA directly from Cabernet Sauvignon with no difficulties but now I am trying to extract RNA from Pinot Noir and I can't do it. One possibility is due to the use of a...

24 January 2018 1,910 2 View

How do I choose among L-ornithine, poly-L/D-lysine or laminin coating?

I have used poly-L-lysine coating when culturing primary neuronal cells. Now I am going to start working with stem cells and I have a protocol indicating to use L-ornithine coating. So, is there a...

16 February 2016 6,899 2 View

N2a cell line culture with or without antibiotics?

Hello all, I am starting to work with N2a cell line and I don't know if I should use antibiotics in the culture medium or not. Some papers mention the use and some others don't. Also, I don't know...

27 January 2016 421 4 View

Do you know any publication that mentions neuronal toxicity independent of oxidative stress?

I am analyzing some results and I find no increase in ROS production but cortical neurons die anyway. This is not the first time that our group struggles with these results. It is hard to explain...

24 August 2015 9,387 5 View