I need to know the conditions to separate peptides with similar molecular properties such as Ala-Gly and Gly-Ala using High Performance Liquid Chromatography (HPLC).
Dear Dilan Dissanayake,
In HPLC, Gly-Ala, Ala-Gly, means you will get almost single peak for both, and in this case its's strongly similar hydrophobic peptide so difficult to separate By HPLC.,
Like this similar molecular weight peptides have some basic, acidic residue means we can able to separate., But one attention here, How you got like this peptide? from where? most probably like this situation not came know.,
For your Question, Similar molecular weight peptides able to separate by HPLC but similar molecular weight without have any acidic,basic, hydrophilic nature difference in C- or N- terminal means unable to seperate by HPLC or LC-MS.,
Hope You Clear.,
Good Luck & Regards
R.Selva.....
Dear Dilan Dissanayake,
In HPLC, Gly-Ala, Ala-Gly, means you will get almost single peak for both, and in this case its's strongly similar hydrophobic peptide so difficult to separate By HPLC.,
Like this similar molecular weight peptides have some basic, acidic residue means we can able to separate., But one attention here, How you got like this peptide? from where? most probably like this situation not came know.,
For your Question, Similar molecular weight peptides able to separate by HPLC but similar molecular weight without have any acidic,basic, hydrophilic nature difference in C- or N- terminal means unable to seperate by HPLC or LC-MS.,
Hope You Clear.,
Good Luck & Regards
R.Selva.....
I Agree Selva Chemist reply, HPLC won't detect like this case, and you will not get like this combination of peptides in single system know? even by synthesis and extraction like this case won't come, some isomers will exist.
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