Dilan Dissanayake

7 Questions 16 Answers 0 Followers

Questions related from Dilan Dissanayake

How to perform an out-of sample forecasting in a time series analysis?

I am working on a time series analysis using yearly data on swine population in Sri Lanka from 1937 to 2017. The objective is to find out the best forecasting model out of the four exponential...

12 December 2019 8,074 7 View

How to monitor PKA activity in skeletal muscle cells by Western Blotting?

I would like to monitor cAMP-dependent protein kinase (PKA) activity in differentiated L6 skeletal muscle cell lysate using western blotting. I hope to use the cells treated with Protein Kinase...

04 April 2017 1,526 2 View

Which DMSO solution is the best to prepare inhibitor solutions to be applied on cultured myotubes?

I want to induce blockade with DPCPX and SH58261 (Selective A1 and A2A adenosine receptor inhibitors, respectively) in cultured L6 rat skeletal muscle cells as a part of an inhibitory assay. Both...

03 March 2017 10,070 3 View

Can anybody tell me how I can separate Gly-Tyr and Tyr-Gly with the same m/z using RP-HPLC?

I need to separate a mixture of dipeptides Gly-Tyr and Tyr-Gly using RP-HPLC.

01 January 2015 9,649 7 View

How do I separate peptides with similar molecular properties using HPLC?

I need to know the conditions to separate peptides with similar molecular properties such as Ala-Gly and Gly-Ala using High Performance Liquid Chromatography (HPLC).

01 January 2015 280 4 View

Dairy Microbiology - What should be the average value that I should use in cfu/mL?

I cultured Lactococcuc lactis subsp. lactis on M17 Media and obtained 6 x 106, 5 x 106 and 3 x 106 cfu/mL in three replicates. What should be the average value that I should use?

08 August 2014 1,734 7 View

What is the optimal protein concentration and primary antibody concentration to detect GLUT4 in L6 muscle cells using Western Blotting?

I want to detect GLUT4 translocation into the plasma membrane of L6 rat skeletal muscle cells upon sample treatment. As a test run, I extracted the sample treated cells with lysis buffer (0.1%...

01 January 1970 3,498 2 View