Hi,
I want to analyse the lymphocyte population by flow cytometry, however my staining and cell population dynamics are being affected due to too many tumor cells present in the lung. Is there any way to remove these?
P.S. I can't use lymphocyte enrichment kit as it may remove the cell population of interest as well.
Thanks!
Can not you use CD45 to separate/gate the lymphocytes from tumor cell group? Good luck.
Hi,
the issue is that too many tumour cells lead to clumping of the cells, because of which I don't get enough live events to analyse the population of my interest with flow cytometry. I'm not sure if a 40/80 percoll gradient would help solve this issue?
Thanks!
Hi,
I have worked with tumors and I find that ficoll density gradient centrifugation removes most of the bulk debris and gives u an enriched lymphocyte fraction. However u end up with few cells for analysis. But this is sufficient for flowcytometry. Percoll should function in a similar way. This is the best that u can do. Otherwise u need to purify CD3 cells using MACS.
Best,
Anchana
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining