Riva Verma

6 Questions 8 Answers 0 Followers

Questions related from Riva Verma

Isolation of single cell from human lung tumour tissue?

Hi, I am planning to isolate single cells from human lung tumour tissue. I am wondering what is the best way to enzymatically degrade the tissue to isolate MOST of the cells (since the population...

10 April 2017 3,240 1 View

How can I quantify tumor burden in mouse lungs in a metastatic model of Lewis lung carcinoma?

I am setting up an experimental metastatic model of Lewis lung carcinoma by intravenous injection of LL/2 cells into the tail vein. I am looking for ways to quantify tumor burden in the lungs. I...

28 February 2017 8,117 2 View

Techniques to analyse effect of micro RNA regulation on global protein production?

Hi, I'd like to know about the current techniques used to study genome wide changes in protein production due to micro RNA regulation. I'm familiar with pSILAC, but is there anything else than...

03 August 2016 3,412 0 View

How do I remove overwhelming tumor cells while preparing mouse lung single cell suspension?

Hi, I want to analyse the lymphocyte population by flow cytometry, however my staining and cell population dynamics are being affected due to too many tumor cells present in the lung. Is there any...

03 June 2016 900 3 View

Is the lung tumour in Lewis Lung Carcinoma model (formed upon subcutaneous injection) a primary tumour?

Apart from the subcutaneous tumour, is the lung tumour also primary or metastatic? If metastatic, does it still represent actual lung tumour?

03 February 2016 564 3 View

Are there well known/established NETosis markers in Neutrophils/WBCs?

I'm interested to know if there are any well known markers to study NETosis in neutrophils using IF?

16 August 2015 6,168 5 View