Hi, I am looking for a protocol and advise about purification of hepatocytes from cancerous tissues samples?
Best and easy way is to isolate hepatocytes from cancerous tissue is to use magnetic beads coated with Glutamyltranspeptidase. This the marker for hepatocyte isolation. The beads are available with DYNABEADS company.
Please check attached paper, it works out well. But little laborious.
Hello
agree with Sameer Mahmood , Has given you a good advice
Look at paper here:
http://cancerres.aacrjournals.org/content/68/8/2641.full
http://link.springer.com/article/10.1007%2FBF02722998
http://www.experimental-surgery.de/page8/page9/
http://clincancerres.aacrjournals.org/content/10/1/202.full
http://www.biomedcentral.com/1471-230X/13/17
http://link.springer.com/article/10.1007/BF02631278
http://dev.biologists.org/content/early/2006/10/04/dev.02589.full.pdf
http://www.nature.com/labinvest/journal/v86/n12/full/3700479a.html
http://dev.biologists.org/content/133/21/4269.full
Hope help you
Good Luck
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