Waheed Ur Rahman

6 Questions 6 Answers 0 Followers

Questions related from Waheed Ur Rahman

Are gangliosides present on monocytes surfaces ?

I would like to know if any of the following Gangliosides are present on the cell surfaces of enlisted cell lines. Gangliosides : GM1, GD1a, GD1b, GT1b & GQ1b Cell lines :HLAC-78, THP-1,...

07 August 2020 8,300 3 View

How to sequentially detach fibroblast and human primary epithelial cells?

I have human nasal primary cells (which is a mixture of fibroblasts and epithelial cells) grown in a flask. I am interested only to detach epithelial cells, leaving the fibroblast on the flask,...

27 November 2019 3,484 0 View

What buffer should I use to counteract the fall of the pH while using sialic acid in competitive receptor binding activity?

I am checking the receptor binding activity of a bacterial toxin in the presence of free sugars (e.g sialic acid) using HNKCM buffer (10mM HEPES (pH 7.4) 140mM NaCl 5mM KCl 2mM CaCl2 and 2mM...

18 July 2019 6,741 1 View

I need suggestions for purification methods of Rtx family toxins

Could someone suggest me any general purification method of Rtx toxin from liquid culture,As I am trying to purify one of the Rtx toxins through Hydrophobic Chromatography but I am getting a high...

10 June 2017 8,192 5 View

How can I improve transformation efficiency ?

I have tried preparing genomic library construction from MDS69 strain of E.Coli, transformed with Keio genomic DNA (blunt end) using the PSG1143 vector. But unluckily the transformation efficiency...

16 December 2015 1,252 3 View

How can I perform ethanol precipitation of 300 ul TE suspended soil DNA ?

Hi I have soil DNA extracted via Soil DNA extraction kit, but after running on Gel I couldn't get any fragment. Now I want to precipitate it with Ethanol prior to amplification . Final suspension...

15 October 2015 1,063 2 View