How to complete digestion step of Kjeldahl method?

We are currently heating 20 mL 95-98% H2SO4, 2 Kjeldahl tabs (each are 1.5 g K2SO4 and 7.5 mg Se), and 1.0 gm protein sample (fish). We have continued the heating process to upwards of 4 hours....

21 December 2020 5,480 3 View

Is an MRes or a skills based MSc better for moving onto a PhD?

I aim to do a PhD in the future (on the general topic of ecology and biogeography) and would like to do a masters before this to make myself a more competitive candidate. However, I am pretty...

03 October 2020 6,680 2 View

Can I replace Sigmas BrdU ELISA kit components with DIY reagents?

Hello everyone! I regularly use the Sigma BrdU cell proliferation kit for my cell assays, and due to the nature of the kit I always run out of some reagents before others. This means I keep...

09 May 2019 1,512 3 View

Trying to run RT-PCR for PDCD4 gene in human serum, can't get any values. Checked primers and they seem okay, any suggestions?

We are having difficulty detecting PDCD4 in serum. We have ordered an ELISA to attempt this, but have not tried it yet. Afraid something is wrong with serum.

08 January 2018 4,637 3 View

Anyone culturing CTLL-2's? Cell density tips?

11 February 2016 8,501 2 View

How to quantify protein concentration......?

I have carried out some nanoparticle treatments on a set of cells, which I plan to use for western blot. Of 7 treatments, one set of cells has turned red, while another dark purple. I think this...

18 January 2016 5,512 7 View

Is anyone using Abcam Anti-CD40 ab13545 for Western blotting?

I have just begun western blots looking at CD40 in immune cell pull downs. The antibody daasheet states the band will be apparent at 41kDa, although the predicted size is 34kDa. I am not too...

24 June 2015 9,594 2 View

Is it possible to use the same primary antibody from different species on the same blot and then use the different secondaries to visualize?

I am using the LiCor Odyssey to visualize my blots. I am trying to decide which primary antibody is the best to use for future experiments. One primary is mouse and the other is rabbit, I have 2...

21 January 2015 9,938 10 View

Series of Tau antibodies for Western blot analysis in human tissue that will span the entire tau protein?

I am looking to find a series of commercially available antibodies for western blot analysis on human tissue covering the expanse of the tau protein (i.e. antibodies detecting the 6 isoforms- both...

27 October 2013 4,469 2 View

I am having issues with my exogenously expressed membrane protein smearing on SDS PAGE gel.

Has anyone had issues with protein band smearing? I reduce the amount of sample I load on the SDS PAGE gel, but all that changes is the intensity of the smear. My protein standard runs normally. I...

26 March 2012 988 18 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View