We are currently heating 20 mL 95-98% H2SO4, 2 Kjeldahl tabs (each are 1.5 g K2SO4 and 7.5 mg Se), and 1.0 gm protein sample (fish). We have continued the heating process to upwards of 4 hours. However, the solution never boils and does not turn clear. Is that okay? Or are we doing something wrong in this process?
Asad Nawaz
Hello Kralik,
Make sure you are using heater not a heating water bath.
Fish protein takes more time to digest. I used protein from bones, skin, meat and scale of fish, it took more time up to 8 hours.
during boiling, it will turn black first and later it will turn blue for fish protein. Endpoint: there comes no black smoke, which will indicate the complete digestion.
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