I want to perform halophility assay to assess the growth pattern of my bacterial strains at different salt concentrations. Can someone kindly share a research article demonstrating reference OD values for comparison? Is there any limit exists like if absorbance value is >0.3 then it shows bacterial are halophiles and if less then they are not?
In the bacterial halophility assay, the reference absorbance values can vary depending on the specific experiment and the bacterial species being tested. It's best to consult the literature or protocol for the assay you're using to determine the appropriate reference absorbance values.
Sure, in the bacterial halophility assay, the reference absorbance values typically range from 0.1 to 0.3. These values serve as a baseline to compare the absorbance of the bacterial cultures under different salt concentrations.
Babatunde Daniel Ayomide Thankyou! yes, I have consulted different papers relevant to my species but they only show trends of OD at different salt levels. You have mentioned 0.1-0.3 base values. Can you kindly share its reference for convenience?
https://core.ac.uk/download/pdf/268085221.pdf
Check that out…
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