Amido black is the nice method to follow for Laemmli buffer. I attached an image of used for protein quantification. I wish this method can help you. Actually, it is relative quantification. If you want exact quantification(how much micro gram) you can use the known concentration of BSA dissolved in Laemmli Buffer along with your samples. Quantify using image J software. All the best. 

Hi Sahu,

It is difficulty to quantify exact amount of your protein in laemmli buffer because of the bromophenol blue and other stuff. 

Same question was discussed before please check the below link

https://www.researchgate.net/post/How_to_quantitate_protein_extracted_in_Laemmli_buffer

You could not get the absolute quantity of your protein with laemmli buffer. A suggestion is to use a known BSA standard and run it in different quantities and do the same thing as your protein. To run the SDS-PAGE and quench by image software. To make your protein intensity fit the BSA standard curve, by that, maybe you could get a proper answer.