CHANDRA SHEKHAR DASARI

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Questions related from CHANDRA SHEKHAR DASARI

Has anyone used restriction enzymes for cloning which produce one sticky end and one blunt end?

I only have two possible restriction sites for cloning an insert into my vector. One end is a sticky end (bglII) and the other end is a blunt end (HPA1or KSPA1). I have never done a blunt end...

23 January 2015 253 9 View

MMP-2 bands are giving streak lines across the gel.

I attached the zymogram image. The top line is MMP-9 giving distinct band. But the MMP2 band is like a straight line across the gel both latent and active forms even in the marker lane. Could any...

07 April 2014 9,302 5 View

What are the dot particles in my LNCaP cell culture?

I found some dot particles after reviving. They have not washed away after 2 to 3 passages. The media colour is not changing even though the particles are huge. The particles are not moving. I...

20 March 2014 5,803 10 View