Parul Sahu

6 Questions 11 Answers 0 Followers

Questions related from Parul Sahu

Hello anyone from New Delhi working in "Cardiovascular disease in animal model"?

I am planning few animal experiment and would like to know the required instruments like ECG and Doppler studies.I would like to discuss , thanks in advance

12 October 2017 7,469 2 View

What to do if we get higher ct value of house keeping genes in qPCR ?

I have reconstituted the RNA from RNA stable tube biomatrica catalogue number 93221001.The 260/280 was 1.81 while 260/230 was 2.The ct value for GAPDH is 27 .Will this value is acceptable.What...

25 August 2017 3,382 6 View

How to design circular RNA primers?can we add extra bases at the 5' end in order for adjusting there Tm

During designing convergent primers we always add extra bases at the 5' end but circular RNA primers are divergent.What criterias should be taken while designing circular RNA primers

30 January 2017 4,017 5 View

What is the procedure for detecting gfp contained parasite in mice blood?

I have injected mice intraperitoneal with gfp plasmodium berghei. I have to confirm the presence of gfp. Can anybody help with the appropriate method for the detection.

10 April 2015 8,342 3 View

Is there any protocol for long term storage of sporozoites of plasmodium berghei ?

Like mammalian cells can be freeze down and use further in future, in the same manner is there any protocol which define the storage and further usage of sporozoites.

30 March 2015 5,299 3 View

What is the concentration of PBS solution used in MTT assay?

PBS concentration in MTT assay

21 July 2014 6,123 4 View