Parul Sahu

9 Questions 16 Answers 0 Followers

Questions related from Parul Sahu

Hello anyone from New Delhi working in "Cardiovascular disease in animal model"?

I am planning few animal experiment and would like to know the required instruments like ECG and Doppler studies.I would like to discuss , thanks in advance

12 October 2017 7,536 2 View

What to do if we get higher ct value of house keeping genes in qPCR ?

I have reconstituted the RNA from RNA stable tube biomatrica catalogue number 93221001.The 260/280 was 1.81 while 260/230 was 2.The ct value for GAPDH is 27 .Will this value is acceptable.What...

25 August 2017 3,438 6 View

How to design circular RNA primers?can we add extra bases at the 5' end in order for adjusting there Tm

During designing convergent primers we always add extra bases at the 5' end but circular RNA primers are divergent.What criterias should be taken while designing circular RNA primers

30 January 2017 4,052 5 View

Is cd 31 a marker of endothelial progenitor cells?

cd31 expresses in mature endothelial cells, but does it expresses in endothelial progenitor cell? cd309 express at which stage in endothelial?

23 December 2016 6,750 4 View

I am interseted for isolating microparticles/microvesicles from blood serum/plasma,Can anybody suggest me better kit for the same?

Kindly send me appropriate catalog number and would like to know about your experience for the same

20 July 2016 6,620 5 View

How do I measure a protein present in Laemmli Buffer?

i have tried BCA and 280nm reading at nanodrop but i couldn't get appropriate results.pls tell me the exact procedure for using infinte m200 nanoquant of teccan,for estimating protein at 280nm

19 October 2015 2,512 3 View

What is the procedure for detecting gfp contained parasite in mice blood?

I have injected mice intraperitoneal with gfp plasmodium berghei. I have to confirm the presence of gfp. Can anybody help with the appropriate method for the detection.

10 April 2015 8,393 3 View

Is there any protocol for long term storage of sporozoites of plasmodium berghei ?

Like mammalian cells can be freeze down and use further in future, in the same manner is there any protocol which define the storage and further usage of sporozoites.

30 March 2015 5,345 3 View

What is the concentration of PBS solution used in MTT assay?

PBS concentration in MTT assay

21 July 2014 6,171 4 View