Vincent Chiang

3 Questions 20 Answers 0 Followers

Questions related from Vincent Chiang

What are the suitable blocking and fixation (inactive) buffer composition for bacterial flow cytometry analysis?

I am going to use flow cytometry to analyze the bacterial surface protein. Does anyone know about the best blocking and the fixation buffer ingredient for bacterial on this experiment? Thank you.

01 October 2015 4,398 1 View

Is there anything wrong in the coloning methods for protein overexpression?

I used NcoI and XhoI for the recombinant protein construction in pET28, and I found it had no any frame shift when simulate construction map in SnapGene software. The problem is I always cannot...

04 April 2015 8,240 14 View

Does anyone have experience on Epitope prediction (both T & B cell)? What is your recommendation?

Epitope prediction have many tool online, but which one will be a generally suitable for polycolone antibody production (maybe for western blot)?

21 March 2013 402 4 View