How do I isolate protoplast in wheat?

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Which is best kit to study the interaction of protein and RNA from tissue sample?

Hello, I am interested in studying the interaction between RNA and proteins, specifically focusing on a particular protein and its corresponding regulatory gene, in mice aortic tissue. Could you...

28 November 2023 4,055 0 View

How to correct error in MO analysis of a dimer in Gaussian ?

In Gaussian, I am trying to do the MO analysis of a compound which is two metal centered having same organic moieties on both the metal. But, I am getting the LUMO which is appearing on only one...

24 January 2023 2,930 2 View

Hello Researchers, is there any alternative for MinPET software for processing of EPMA data?

MinPET software works only below win7 32 bit operating system. So we are unable to use it in higher operating system.

18 October 2022 394 0 View

Flea Beetles control in Cauliflower?

Dear connection, I need your help reagarding 100% control of Flea Beetles in Cauliflower crops. Thanks and regards Dhananjay Yadav

24 January 2022 9,539 3 View

How do we identify a mineral using SEM-EDX data i.e elements in wt%. Is there any atlas, EDX- library or guide for mineral identification?

Data is provided in terms of wt% and Atomic wt% of elements. So how do we identify the mineral?

08 September 2021 3,608 1 View

Can somebody suggest any software related to Laser parameteric analysis?

I would like to understand the different parameters of laser (pulsed laser) and their effect. In my case laser specification is a Q-switched Nd: YAG laser (200 mJ; 30 Hz; 6-9 ns). I am using this...

12 August 2021 8,550 3 View

Does anyone have state estimation MATLAB code for 3-phase unbalanced distribution network?

Does anyone has state estimation code using WLS for a 3-phase unbalanced IEEE 13 bus system. Is there any relation with r/x ratio to its convergence. Please help me. Thank you in Advance.

25 June 2021 8,838 1 View

How do you go about isolating the source of electrical noise on a ephys rig?

I am very new to electrophysiology. I have tried to the model cell and the noise does not go away. I have foiled all the wires that come out of the rig. I have tried unplugging and replugging the...

09 June 2021 5,162 1 View

In distribution system what are the methods which can be applied for finding Fault Location?

Using SCADA system what data parameters and best method (formula) may be utilized for fault location.

30 May 2021 7,415 9 View

How to modify the Y/Z bus matrix for 3 phase lines between two bus and how to do load flow analysis in distribution system?

How the distribution system power flow analysis is done for each phase of the line including various components of distribution system? Thank you in advance for your help.

31 August 2020 2,447 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View