Dhananjay Dhokane

4 Questions 6 Answers 0 Followers

Questions related from Dhananjay Dhokane

Coating of gRNA and Cas9 protein with gold/tungsten for gene gun mediated transformation?

Hello, Can anyone please provide me a detail protocol for coating gRNA and Cas9 protein onto gold/tungsten particles for particle bombardment in plants. Thanks!

29 March 2017 5,295 8 View

How do I isolate protoplast in wheat?

May any one suggest me a simple and an efficient protocol for protoplast isolation in wheat??

23 April 2015 3,719 3 View

What is the fate of a vector backbone once the gene of interest gets integrated in a plant genome?

Plant transformation.

05 April 2014 5,643 2 View

What is the fate of the vector backbone once the gene of interest gets integrated into the plant genome?

see above

04 April 2014 2,043 1 View