Hello al
l I have an Epstein-Barr Virus- (EBV-) Immortalized Lymphoblastoid Cell Lines (LCLs) which grow very slow. the media I using is 10%RMPI or DCCM1
how can I improve my growth rate?
thanks
Leonor
Hello Leonor Daniel
I would like you to refer to the research article below.
Summary:
Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. The virus mainly infects B lymphocytes and could immortalize and transform the cells into EBV-bearing lymphoblastoid cell lines (LCLs). Limited studies have been focused on characterizing the surface marker expression of the immortalized LCLs. This study demonstrates the generation of 15 LCLs from sixteen rheumatoid arthritis (RA) patients and a healthy volunteer using B95-8 marmoset-derived EBV. The success rate of LCL generation was 88.23%. All CD19+ LCLs expressed CD23 (16.94-58.9%) and CD27 (15.74-80.89%) on cell surface. Our data demonstrated two distinct categories of LCLs (fast- and slow-growing) (p
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