What is the smallest Archaeological feature size that has been successfully analyzed using either LIDAR or Satellite Image analysis?

I am considering the viability of using AI in conjunction with either LIDAR based Digital Elevation Models or Object based image analysis of Satellite Imagery to predictively locate Inuit...

25 October 2020 9,525 2 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

How do anthropogenic pressures influence and will influence coastal groundwater according to the climate and its evolution?

Groundwater is of utmost importance in Haiti. It is the main source of drinking water for the Haitian population. The degradation of the quality of this resource is a major issue in the context...

29 June 2020 8,626 6 View

No title

Whether for a dissertation, a thesis or any other research work, there will be a problem in which it is clearly posed what is causing the problem and which imposes on the researcher the need to...

29 June 2020 4,902 9 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

How to purify large amounts of plasmid after restriction digest?

03 November 2019 2,686 4 View

What is the suitable and updated seismic attenuation models for Japan/Philippines?

We are working on different seismic studies both for research use and consultancy works. We've learned that Fukushima and Tanaka attenuation models are already outdated (model we used before)....

17 December 2018 7,970 2 View

Data of Propane/R290 and IsoButane/R600a Solubility in POE lubricant oils?

We need the test data of propane/R290 and isobutane/R600a solubility in POE lubricant oils. The data will be used to model the refrigerant quantity dissolved in compressor oils ( www.refriglab.com...

20 May 2018 9,451 1 View

How to choose between publishing and patenting?

Generally, academic researchers focus on publishing on public journals while company researchers are more interested in patenting. Sometimes, it is hard to choose between publishing your good...

20 April 2018 5,585 8 View

Shall we publish on online journals?

Most of us have the experiences being invited to write or review a paper for a new online journal. It looks that it is generally much easier and quicker to publish on the new online journals. Do...

19 April 2018 807 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View