What is the smallest Archaeological feature size that has been successfully analyzed using either LIDAR or Satellite Image analysis?

I am considering the viability of using AI in conjunction with either LIDAR based Digital Elevation Models or Object based image analysis of Satellite Imagery to predictively locate Inuit...

25 October 2020 9,525 2 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

How do anthropogenic pressures influence and will influence coastal groundwater according to the climate and its evolution?

Groundwater is of utmost importance in Haiti. It is the main source of drinking water for the Haitian population. The degradation of the quality of this resource is a major issue in the context...

29 June 2020 8,626 6 View

No title

Whether for a dissertation, a thesis or any other research work, there will be a problem in which it is clearly posed what is causing the problem and which imposes on the researcher the need to...

29 June 2020 4,902 9 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

How to purify large amounts of plasmid after restriction digest?

03 November 2019 2,686 4 View

What is the suitable and updated seismic attenuation models for Japan/Philippines?

We are working on different seismic studies both for research use and consultancy works. We've learned that Fukushima and Tanaka attenuation models are already outdated (model we used before)....

17 December 2018 7,970 2 View

Data of Propane/R290 and IsoButane/R600a Solubility in POE lubricant oils?

We need the test data of propane/R290 and isobutane/R600a solubility in POE lubricant oils. The data will be used to model the refrigerant quantity dissolved in compressor oils ( www.refriglab.com...

20 May 2018 9,451 1 View

How to choose between publishing and patenting?

Generally, academic researchers focus on publishing on public journals while company researchers are more interested in patenting. Sometimes, it is hard to choose between publishing your good...

20 April 2018 5,585 8 View

Shall we publish on online journals?

Most of us have the experiences being invited to write or review a paper for a new online journal. It looks that it is generally much easier and quicker to publish on the new online journals. Do...

19 April 2018 807 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View