I need one buffer that can be compatible in PCR amplification.
Some buffer with good elution efficiency but not compatible in PCR
1) 1M phosphate buffer
2) dNTP solution,
3) NaCl
etc.
I would try adding the NPs without eluting the DNA (just wash them in TE). The PCR denaturation steps will probably liberate PCR amplifiable DNA.
Dear Geoff,
thank you very much for your answer. I am interested in your answer. Do you test it without elution？
We do not recommend to use NPs for PCR directly，becasue，the enzyme may be adsorbed onto NPs surface，resulting in inhibitor of PCR. That's why we interested in release DNA from NPs， before PCR.
Thanks again for your answer.
I have not tested this personally. But it is what I would do, and its quick to try! If you get no PCR band (while all the controls work OK) then you are still at square 1.
The NPs are likely to stay at the bottom of the tube, so I would carry out the initial denaturation step, and at the end of that step, pause and quickly vortex the tube. Then continue.
Good luck!
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