I do some research about LncRNA, which is located in nuclear and i want to knock down the expression by transfected the antisense oligonucleotide. Who tell me how to design and some tips about this experiment.
Here you find useful information for designing antisense oligonucleotides:
http://www.genelink.com/oligo_modifications_reference/OMR_mod_category_design.asp?mod_sp_cat_id=17 and
http://eu.idtdna.com/pages/decoded/decoded-articles/core-concepts/decoded/2015/07/17/a-new-renaissance-for-antisense-in-the-era-of-lncrna
Cheers, Christian
However, first you have to find a target for knockdown expression and target should be 5' or 3' end to target the RNAi some regions may not be effective. Then design ologos according to antisense, which is complementary to your target using IDTDNA article. Then you can design LncRNA according to effective regions.
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