Hi,
For 28 day cell culture I use a fibrin hydrogel, which contains aprotinin to delay degradation by my cells.
However, for certain assays (for qPCR and Extracellular matrix) I need to extract cells or produced ECM. In order to extract those, I should dissolve my fibrin and/or remove it.
I tried different concentrations of Trypsin-EDTA and incubation times ( and max 0.25% and over an hour). Still my fibrin remains stable and cells nor ECM come loose, but remain integrated with the fibrin gel.
Leaving aprotinin out of the fibrin, would make my samples degrade too soon to perform measurements after weeks of culture I guess..
Curious to any thoughts or ideas, thanks!
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