How to calculate the Water Stress Index for a basin?

Water Stress Index (WSI) and Environmental water requirements (EWR). Thank you.

06 December 2020 904 3 View

What is the best method to sterilize hydroxyapatite nanoparticles?

Powder insoluble

08 November 2020 509 3 View

Where can I find a downloadable dataset of intoxicated face?

I need about 500+ pictures of intoxicated face (of drunk or unsober people) to train a recognized system. Can you give me some downloadable links?

19 September 2020 239 2 View

Does DOLS deal with cross sectional dependence?

Hello researchcers Can you help me to understand that the DOLS model is suitable for cross sectional dependence panel data or not. The sample of my research is CSD through individuals...

28 August 2020 2,964 5 View

How do I measure collagen deposition in asthmatic mouse lung using ImageJ?

Hello All I am working on allergic asthma and hyper-responsiveness in mouse exposed to Aspergillus fumigatus. I am trying to measure collagen deposition using the ImageJ software but i don't know...

02 March 2020 6,830 3 View

Can I use fixed angle centrifuge for plasma fractioning to extract microRNA?

I am going to collect venous blood samples for the thesis project. and my inquiry is, does fixed-angle centrifuge suitable to be used for plasma fractioning for miRNA extraction? especially, K2...

27 January 2020 7,432 2 View

Cell culture of type k562 and daudi ?

What causes contamination when culturing these two types of cancer cells (leukemia) k562, Daudi?

15 September 2019 7,236 3 View

Questions about the transfection?

I made transfection of some cell lines using siRNA and cationic polymer, I am sure the polyplexes were formed (NP ratio of polyplexes were determined by the agarose gel) but the transfection...

07 August 2019 9,824 5 View

Does anyone know a specific staining for lead in fish tissue samples?

I need to stain lead (Pb) in paraffin-embedded sections of fish liver. Samples were previously fixed in 10% Neutral Buffered Formalin. I am struggling to looking for a specific staining for lead...

02 May 2019 8,005 3 View

Is there an open dataset with Multitrait-multimethod design available to use?

Hi all, I'm a graduate student at the University of Illinois and I'm learning how to conduct exploratory structural equation modeling (ESEM) with multitrait-multimethod design. Is there an...

29 January 2019 934 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View