Hi, I'm a PhD student working to develop electoconductive, electrospun scaffolds for cardiac tissue engineering applications. I have been seeding cells onto these scaffolds but having major issues during cell culture and live/dead assays, immuostaining, etc. Basically while I'm aspirating out media, PFA, PBS, live/dead kit, etc. it ends up sucking up parts of the scaffolds too because the fibers are so small. I tried using a pipette gun instead of aspirating tube and it helped but not enough. Other than just spinning thicker samples, what else can I do? The fibers are 1:1 PCL and gelatin. The cells are NIH 3T3 but I will be using iPSC-CMs soon so I want to get this issue fixed before I do. Please let me know if you need any more info, and thanks in advance!
You can try increasing the stiffness and stability of the scaffold so that these are not so fragile. Otherwise do not use an aspirator or pipette gun as they use more mechanical force. Try simple pipetting very gently to avoid touching the scaffold.
previously when I cope with gel it also get aspirated. you can use a tip or sth else to press it
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