How do I avoid sucking up electrospun scaffolds during cell culture?

Hi, I'm a PhD student working to develop electoconductive, electrospun scaffolds for cardiac tissue engineering applications. I have been seeding cells onto these scaffolds but having major...

04 December 2020 6,672 3 View

Any recommendation on transfection reagent for siRNA treatment of C6/36 cells?

I'm looking for effective transfection reagent for siRNA treatment of C6/36 cells. I've used DharmaFect and Lipofectamine2000, but it gave transient knock-down of the protein or ineffective...

04 November 2019 5,082 5 View

How to etching sol-gel metal-oxide selectively?

18 May 2017 5,006 3 View

How to inactivate bacteria without affecting the DNA?

I have specifically 3 bacteria in mind : 1. Bacillus cereus 2. Legionella Pneumophila 3. staphylococcus aureus I want to heat-treated them so they will be safe to handle without affecting the DNA...

21 March 2016 5,807 2 View

What is the best method to sterilize lysates which potentially contain peptide?

I'm doing a screening on lysates with unknown properties against virus on cell culture. I need to make sure the lysates are sterile so as not to contaminate my cell cultures. What is the best...

22 November 2015 4,267 3 View

How much the band gap energy of Molybdenum trioxide is?

22 December 2014 5,060 9 View

Can anyone help me with secondary structure probes for real-time pcr?

I am designing primers for real-time pcr for both dyes and probes based chemistry. But I am having problems designing the probe. The probes have secondary structures that went beyond the...

30 April 2014 7,318 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View