Hi Heru

To make it really quick you can measure a protein content with a standard Proteinassay,

Bradford, BCA and/or E280 to fix the total amount of protein. Maybe you do this already, than you can analyze the samples with ninhydrin-assay, this stains only amino acids and short peptides. This will give you a good overview about the distribution of Amino Acids and Proteins and you can correlate them

To get a better qualitative proof you can run a quick TLC with a system to separate Amino Acid, TLC can be principally measured and quantified too....

Thank you for your answer Frank, 

yes we already done bradford test, do you know to measure kind of FAA with simple method than GC-MS. It will be really help for our students since our laboratory didn't support to do GC-MS, 

Thank you

Hi Heru MS will not help you much, because usually they don't have a good reproducability for quantitative samples, the molecular mass that you estimate is a good qualitative information but not easy to quantify.

This method looks interesting:

http://hydh.hbstl.org.cn/uploadfiles/4HBO7XZP1300350157052.pdf

They deliver  a good description too ...

Hi Frank

Thank you, that really help our project

Good Luck, maybe I hear from you after project finishing, would be interesting

we hope so..