How can I isolate and culture protozoa from shrimp?

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Can linear DNA expressed in Enterobacteria?

Hi, I transformed a PCR product containing GFP from pGFPuv without Lac promoter (change it with 50bp homologous arm for pRED/ET recombination) into Enterobacteria by electroporation. I expected to...

11 November 2020 8,714 3 View

100kb Plasmid without repA?

Dear all, I assembled MiSeq reads using SPAdes. The sample is an endosymbiont bacteria. I found a long contig, almost 100 Kb, good coverage. At first, I thought it was part of my target bacteria...

07 May 2016 1,149 1 View

RMSD data from Molecular Dynamics

I am facing weird molecular dynamics data. The mean RMSD for 312 K lies below the mean RMSD for 310, this seems to be impossible since with higher temperatures, the RMSD should be greater. Any...

25 November 2012 1,163 15 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View