How do deng and rojas primer differ in their function when used in PCR amplification of begomovirus genes?

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Sibnarayan Datta

Dear Kamesh,

Deng A/B is a single degenerate primer pair to amplify the precoat/movement protein encoding region (V2) of the Begomovirus genome. However, Rojas primers are referred to a number of degenerate primers which can be used to amplify different genetic regions of the Begomovirus genome. So decesion to use primers must be based on the target region of the genome that you want to amplify. If you need more info, let me know.

best wishes,

Kamesh Krishnamoorthy

@Dr Sibnarayan datta..Thanks a lot for the information sir...I need some more information regarding this..could you provide me your email id for the same..thanking you

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