We will trying ti isolate marker positive extracellualr vesicle(EV), from pre-enriched EV sample using antibody conjugated bead.
The used bead for antibody conjugation is aldehyde/sulfate or carboxylated bead.
We confirmed that EV attached to these bead thorough remaining EV amount in supernatant (using protein amount) and bead based ELISA.
Now the problem is how disassemble or detach these EV and antibody-conjugated bead complex.
The commercial product just lysis the EV in state of EV and bead complex or just used in FACS.
In researchgate question section, pH adjustment (low pH) could be used it, but it require additional wash step (maybe ultracentrifugation step).
Is there any method to disassemble the EV-antibody conjugated bead complex, without additional wash step? (except the bead elimination step, the low speed centrifugation is enough to do)